Nnsolid phase dna synthesis pdf files

We report chemistry suitable for the solidphase synthesis of dnaencoded libraries with an unusually high level of structural diversity. However, an oxadiazoleforming variant of the ugi reaction caused dna depurination, requiring a more stable hexathymidine dna for encoded library synthesis. Pdf solid phase versus solution phase synthesis of. The ugi, ugiazide, and groebkeblackburnbienayme reactions are welltolerated by dna on the solid phase and show a broad scope. This synthesis method is difficult to control resulting in agglomeration of the enm and is thus rarely used and will not be discussed further 32. Although dna exhibits limited solubility in nonaqueous solvents, its immobilization on a solid phase can be exploited to access such environments. Isocyanide multicomponent reactions on solidphasecoupled. With a solidphase chemistry approach, a large existing body of known chemical transformations becomes accessible to dnaencoded synthesis. High efficiency and throughput increased simplicity and speed the reaction can be driven to completion and high yields through the use of excess reagent. In chemistry, solid phase synthesis is a method in which molecules are covalently bound on a solid support material and synthesised stepbystep in a single reaction vessel utilising selective protecting group chemistry. Solid phase synthesis of 5 triantennary nacetylgalactosamine conjugated antisense oligonucleotides using phosphoramidite chemistry.

Solidphase synthesis is carried out on a solid support held between filters, in columns that enable all reagents and solvents to pass through freely. Solid phase peptide synthesis part i applications of bocbzl strategy g bor mez research group of peptide chemistry hungarian academy of sciences powerpoint ppt presentation. Dnabinding polyamides are synthetic oligomers of pyrroleimidazole units with high specificity and affinity for doublestranded dna. Solidphase synthesis of dnaencoded libraries via an. In this method, building blocks are protected at all react.

Solid phase versus solution phase synthesis of heterocyclic macrocycles article pdf available in molecules 181. Aristolochic acid aa is a known nephrotoxin and potential carcinogen, which can form covalent dna adducts after metabolic. To increase their synthetic diversity, we report a mild methodology based on 4methyltrityl mtt solid phase peptide synthesis spps, whose building blocks are more accessible than the standard fmoc and boc spps ones. The solid vapor synthesis method is technically classified as a vapor aerosol phase synthesis and involves the reaction between solid and gas precursors. Spps is more efficient, faster and the purification is easier omethod often used in chemistry and biochemstry. Solid phase peptide synthesis, strategies and resins.